Information de reference pour ce titreAccession Number: | 00078401-201802000-00024.
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Author: | Li, Zhong a; Sakamuru, Srilatha b; Huang, Ruili b; Brecher, Matthew a; Koetzner, Cheri A. a; Zhang, Jing a; Chen, Haiying c; Qin, Cheng-feng d; Zhang, Qing-Yu a,e; Zhou, Jia c; Kramer, Laura D. a,e; Xia, Menghang b; Li, Hongmin a,e,*
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Institution: | (a)Wadsworth Center, New York State Department of Health, 120 New Scotland Ave, Albany, NY 12208, USA (b)National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD 20892, USA (c)Chemical Biology Program, Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555, USA (d)Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, People's Republic of China (e)Department of Biomedical Sciences, School of Public Health, University at Albany, PO Box 509, Empire State Plaza, Albany NY 12201, USA
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Title: | |
Source: | Antiviral Research. 150:217-225, February 2018.
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Abstract: | Many flaviviruses, such as Zika virus (ZIKV), Dengue virus (DENV1-4) and yellow fever virus (YFV), are significant human pathogens. Infection with ZIKV, an emerging mosquito-borne flavivirus, is associated with increased risk of microcephaly in newborns and Guillain-Barre syndrome and other complications in adults. Currently, specific therapy does not exist for any flavivirus infections. In this study, we found that erythrosin B, an FDA-approved food additive, is a potent inhibitor for flaviviruses, including ZIKV and DENV2. Erythrosin B was found to inhibit the DENV2 and ZIKV NS2B-NS3 proteases with IC50 in low micromolar range, via a non-competitive mechanism. Erythrosin B can significantly reduce titers of representative flaviviruses, DENV2, ZIKV, YFV, JEV, and WNV, with micromolar potency and with excellent cytotoxicity profile. Erythrosin B can also inhibit ZIKV replication in ZIKV-relevant human placental and neural progenitor cells. As a pregnancy category B food additive, erythrosin B may represent a promising and easily developed therapy for management of infections by ZIKV and other flaviviruses.
Highlights:
* We identified erythrosin B as a potent inhibitor for flavivirus protease.
* Erythrosin B inhibited the DENV2 protease via a non-competitive mechanism.
* Erythrosin B interferes the interactions between viral NS3 and its co-factor NS2B.
* Erythrosin B is a potent inhibitor for Zika virus and Dengue virus.
(C) 2018Elsevier, Inc.
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Author Keywords: | Flavivirus; Zika virus; Dengue virus; Antiviral; Protease inhibitor; Erythrosin B.
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Language: | English.
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Document Type: | Articles.
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Journal Subset: | Clinical Medicine. Pharmacology.
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ISSN: | 0166-3542
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DOI Number: | https://dx.doi.org/10.1016/j.ant...- ouverture dans une nouvelle fenêtre
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