Information de reference pour ce titreAccession Number: | 00008563-201206010-00009.
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Author: | Sancenon, Vicente 1; Lee, Sue-Ann 1,2; Patrick, Christina 5; Griffith, Janice 6; Paulino, Amy 5; Outeiro, Tiago F. 7,8; Reggiori, Fulvio 6; Masliah, Eliezer 5; Muchowski, Paul J. 1,2,3,4,*
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Institution: | (1)Gladstone Institute of Neurological Disease, 1650 Owens Street, San Francisco, CA 94158, USA, (2)Biomedical Sciences Graduate Program, (3)Department of Biochemistry and Biophysics and (4)Department of Neurology, University of California, San Francisco, USA, (5)Department of Pathology and Medicine, University of California, San Diego, La Jolla, CA 92093, USA, (6)Department of Cell Biology, University Medical Center Utrecht, Utrecht, CX 3584, The Netherlands, (7)Cellular and Molecular Neuroscience Unit and (8)Instituto de Medicina Molecular, Instituto de Fisiologia, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal
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Title: | |
Source: | Human Molecular Genetics. 21(11):2432-2449, June 1, 2012.
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Abstract: | The aggregation of [alpha]-synuclein ([alpha]Syn) is a neuropathologic hallmark of Parkinson's disease and other synucleinopathies. In Lewy bodies, [alpha]Syn is extensively phosphorylated, predominantly at serine 129 (S129). Recent studies in yeast have shown that, at toxic levels, [alpha]Syn disrupts Rab homeostasis, causing an initial endoplasmic reticulum-to-Golgi block that precedes a generalized trafficking collapse. However, whether [alpha]Syn phosphorylation modulates trafficking defects has not been evaluated. Here, we show that constitutive expression of [alpha]Syn in yeast impairs late-exocytic, early-endocytic and/or recycling trafficking. Although members of the casein kinase I (CKI) family phosphorylate [alpha]Syn at S129, they attenuate [alpha]Syn toxicity and trafficking defects by an S129 phosphorylation-independent mechanism. Surprisingly, phosphorylation of S129 modulates [alpha]Syn toxicity and trafficking defects in a manner strictly determined by genetic background. Abnormal endosome morphology, increased levels of the endosome marker Rab5 and co-localization of mammalian CKI with [alpha]Syn aggregates are observed in brain sections from [alpha]Syn-overexpressing mice and human synucleinopathies. Our results contribute to evidence that suggests [alpha]Syn-induced defects in endocytosis, exocytosis and/or recycling of vesicles involved in these cellular processes might contribute to the pathogenesis of synucleinopathies.
(C) Copyright Oxford University Press 2012.
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Language: | English.
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Document Type: | ARTICLES.
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Journal Subset: | Life & Biomedical Sciences.
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ISSN: | 0964-6906
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NLM Journal Code: | brc, 9208958
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DOI Number: | https://dx.doi.org/10.1093/hmg/d...- ouverture dans une nouvelle fenêtre
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