Information de reference pour ce titreAccession Number: | 00004348-201209000-00018.
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Author: | Lowe, C E; Dennis, R J; Obi, U; O'Rahilly, S; Rochford, J J
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Institution: | University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, Addenbrooke's Hospital, Cambridge, UK.
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Title: | Investigating the involvement of the ATF6[alpha] pathway of the unfolded protein response in adipogenesis.[Miscellaneous Article]
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Source: | International Journal of Obesity. 36(9):1248-1251, September 2012.
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Abstract: | : The unfolded protein response (UPR) is activated by endoplasmic reticulum stress resulting from an accumulation of unfolded or mis-folded proteins. The UPR is divided into three arms, involving the activation of ATF-6, PERK and IRE-1, that together act to restrict new protein synthesis and increase the production of chaperones. Recent studies have implicated the PERK and IRE-1 components of the UPR in adipocyte differentiation. In this study, we investigate the importance of ATF6[alpha] during adipogenesis using stable knockdown of this protein in the model adipogenic cell line, C3H10T1/2. Reduction of ATF6[alpha] expression by >70% resulted in impaired expression of key adipogenic genes and reduced lipid accumulation following the induction of adipogenesis. In contrast, loss of ATF6[alpha] did not impair the ability of cells to undergo osteogenic differentiation. Overall, our data indicate that all three arms of the UPR, including ATF6[alpha], must be intact to permit adipogenesis to occur.
Copyright (C) 2012 Nature Publishing Group
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Author Keywords: | adipogenesis; unfolded protein response; ATF6[alpha].
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Language: | English.
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Document Type: | SHORT COMMUNICATION.
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Journal Subset: | Clinical Medicine. Life & Biomedical Sciences.
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ISSN: | 0307-0565
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NLM Journal Code: | 7703240
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DOI Number: | https://dx.doi.org/10.1038/ijo.2...- ouverture dans une nouvelle fenêtre
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