Information de reference pour ce titreAccession Number: | 01445408-201101000-00008.
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Author: | Pratt, Margaret M. 1; King, Leon C. 2; Adams, Linda D. 2; John, Kaarthik 1; Sirajuddin, Paul 1; Olivero, Ofelia A. 1; Manchester, David K. 3; Sram, Radim J. 4; DeMarini, David M. 2; Poirier, Miriam C. 1
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Institution: | (1)Carcinogen-DNA Interactions Section, LCBG, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland (2)Integrated Systems Toxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina (3)Clinical Genetics and Metabolism, University of Colorado Denver School of Medicine, The Children's Hospital, Aurora, Colorado (4)Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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Title: | |
Source: | Environmental & Molecular Mutagenesis. 52(1):58-68, January 2011.
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Abstract: | : Three classes of DNA damage were assessed in human placentas collected (2000-2004) from 51 women living in the Teplice region of the Czech Republic, a mining area considered to have some of the worst environmental pollution in Europe in the 1980s. Polycyclic aromatic hydrocarbon (PAH)-DNA adducts were localized and semiquantified using immunohistochemistry (IHC) and the Automated Cellular Imaging System (ACIS). More generalized DNA damage was measured both by 32P-postlabeling and by abasic (AB) site analysis. Placenta stained with antiserum elicited against DNA modified with 7[beta],8[alpha]-dihydroxy-9[alpha],10[alpha]-epoxy-7,8,9,10-tetrahydro-benzo[a]pyrene (BPDE) revealed PAH-DNA adduct localization in nuclei of the cytotrophoblast (CT) cells and syncytiotrophoblast (ST) knots lining the chorionic villi. The highest levels of DNA damage, 49-312 PAH-DNA adducts/108 nucleotides, were found by IHC/ACIS in 14 immediately fixed placenta samples. An additional 37 placenta samples were stored frozen before fixation and embedding, and because PAH-DNA adducts were largely undetectable in these samples, freezing was implicated in the loss of IHC signal. The same placentas (n = 37) contained 1.7-8.6 stable/bulky DNA adducts/108 nucleotides and 0.6-47.2 AB sites/105 nucleotides. For all methods, there was no correlation among types of DNA damage and no difference in extent of DNA damage between smokers and nonsmokers. Therefore, the data show that DNA from placentas obtained in Teplice contained multiple types of DNA damage, which likely arose from various environmental exposures. In addition, PAH-DNA adducts were present at high concentrations in the CT cells and ST knots of the chorionic villi. Environ. Mol. Mutagen. 52:58-68, 2011. (C) 2010 Wiley-Liss, Inc.
Copyright (C) 2011 John Wiley & Sons, Inc.
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Author Keywords: | automated cellular imaging system; immunohistochemistry; BPDE-DNA antiserum; abasic sites; 32P-Postlabeling; Teplice Program.
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Language: | English.
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Document Type: | Research Articles.
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Journal Subset: | Life & Biomedical Sciences. Science.
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ISSN: | 0893-6692
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DOI Number: | https://dx.doi.org/10.1002/em.20...- ouverture dans une nouvelle fenêtre
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