Information de reference pour ce titreAccession Number: | 00005797-201003020-00003.
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Author: | Lappann, Martin 1*; Claus, Heike 1,2; van Alen, Tessa 1; Harmsen, Morten 3,4; Elias, Johannes 1,2; Molin, Soren 3; Vogel, Ulrich 1,2
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Institution: | (1)University of Wurzburg, Institute for Hygiene and Microbiology, Germany. (2)University of Wurzburg, National Reference Laboratory for Meningococci, Germany. (3)Technical University of Denmark, Department of Systems Biology, Denmark. (4)Copenhagen University, Department of Food Science, Denmark.
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Title: | |
Source: | Molecular Microbiology. 75(6):1355-1371, March 2010.
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Abstract: | : Major pathogenic clonal complexes (cc) of Neisseria meningitidis differ substantially in their point prevalence among healthy carriers. We show that frequently carried pathogenic cc (e.g. sequence type ST-41/44 cc and ST-32 cc) depend on extracellular DNA (eDNA) to initiate in vitro biofilm formation, whereas biofilm formation of cc with low point prevalence (ST-8 cc and ST-11 cc) was eDNA-independent. For initial biofilm formation, a ST-32 cc type strain, but not a ST-11 type strain, utilized eDNA. The release of eDNA was mediated by lytic transglycosylase and cytoplasmic N-acetylmuramyl-L-alanine amidase genes. In late biofilms, outer membrane phospholipase A-dependent autolysis, which was observed in most cc, but not in ST-8 and ST-11 strains, was required for shear force resistance of microcolonies. Taken together, N. meningitidis evolved two different biofilm formation strategies, an eDNA-dependent one yielding shear force resistant microcolonies, and an eDNA-independent one. Based on the experimental findings and previous epidemiological observations, we hypothesize that most meningococcal cc display a settler phenotype, which is eDNA-dependent and results in a stable interaction with the host. On the contrary, spreaders (ST-11 and ST-8 cc) are unable to use eDNA for biofilm formation and might compensate for poor colonization properties by high transmission rates.
Copyright (C) 2010 Blackwell Publishing Ltd.
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Language: | English.
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Document Type: | Research Articles.
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Journal Subset: | Life Sciences.
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ISSN: | 0950-382X
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NLM Journal Code: | mom, 8712028
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DOI Number: | https://dx.doi.org/10.1111/j.136...- ouverture dans une nouvelle fenêtre
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