Information de reference pour ce titreAccession Number: | 00000556-200912000-00003.
|
Author: | Estelle, J. *,1; Fernandez, A. I. +; Perez-Enciso, M. *,++; Fernandez, A. +; Rodriguez, C. +; Sanchez, A. *; Noguera, J. L. [S]; Folch, J. M. *
|
Institution: | (*)Departament de Ciencia Animal i dels Aliments, Facultat de Veterinaria, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain (+)Departamento de Mejora Animal SGIT-INIA, 28040 Madrid, Spain (++)Institut Catala de Recerca i Estudis Avancats, 08010 Barcelona, Spain ([S])Genetica i Millora Animal, IRTA Lleida, 25198 Lleida, Spain
|
Title: | |
Source: | Animal Genetics. 40(6):813-820, December 2009.
|
Abstract: | Summary: Despite the economic interest of the fatty acid profile in pigs, no gene has been convincingly associated with this trait so far. Here, the porcine microsomal triglyceride transfer protein (MTTP) gene, which plays a crucial role in the assembly of nascent lipoproteins, has been analysed as a positional candidate gene for a QTL affecting the fatty acid composition that was previously identified on chromosome 8 in an Iberian by Landrace F2 cross. By resequencing a panel of different breeds, a non-synonymous polymorphism in a conserved residue of the lipid transfer domain of MTTP was identified. Association analyses with this polymorphism showed a strong association with the fatty acid composition of porcine fat, much stronger than the QTL effect, in the F2 cross and in a synthetic Sino-European line. In addition, in vitro activity assays in liver protein extracts have shown that this mutation is also associated with the lipid transfer activity of the MTTP protein (P < 0.1). These results suggest that the detected polymorphism is a potential causal factor of the fatty acid composition QTL. There appears to be an interaction between the porcine MTTP genotype and the type of fat source in the pig diet, which would agree with the previous results on the biology of MTTP biology.
Copyright (C) 2009 Blackwell Publishing Ltd.
|
Author Keywords: | fatty acid composition; porcine; protein activity; QTL; SNP.
|
References: | Anderson T.A., Levitt D.G. & Banaszak L.J. (1998) The structural basis of lipid interactions in lipovitellin, a soluble lipoprotein. Structure 6, 895-909.
Bates P.A., Kelley L.A., MacCallum R.M. & Sternberg M.J. (2001) Enhancement of protein modeling by human intervention in applying the automatic programs 3D-JIGSAW and 3D-PSSM. Proteins: Structure, Function and Genetics 45(Suppl 5): 39-46.
Benjamini Y. & Hochberg Y. (1995) Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society: Series B 57, 289-300.
Carlsson A.S., LaBrie S.T., Kinney A.J., Wettstein-Knowles P.v. & Browse J. (2002) A KAS2 cDNA complements the phenotypes of the Arabidopsis fab1 mutant that differs in a single residue bordering the substrate binding pocket. Plant Journal 29, 761-70.
Clop A., Ovilo C., Perez-Enciso M. et al. (2003) Detection of QTL affecting fatty acid composition in the pig. Mammalian Genome 14, 650-6.
Corpet F. (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16, 10881-90.
Daza A., Mateos A., Rey A.J., Ovejero I. & Lopez-Bote C.J. (2007) Effect of duration of feeding under free-range conditions on production results and carcass and fat quality in Iberian pigs. Meat Science 76, 411-6.
Delano W.L. (2002) The PyMOL Molecular Graphics System. DeLano Scientific, Palo Alto, CA, USA. Available at: http://www.pymol.org- ouverture dans une nouvelle fenêtre.
Dyson H.J. & Wright P.E. (2005) Intrinsically unstructured proteins and their functions. Nature Reviews Molecular and Cell Biology 6, 197-208.
Estelle J., Sanchez A. & Folch J.M. (2005) Assignment of the microsomal triglyceride transfer protein large subunit (MTP) gene to porcine chromosome 8. Animal Genetics 36, 354-5.
Estelle J., Mercade A., Perez-Enciso M., Pena R.N., Silio L. & Folch J.M. (2009) Evaluation of FABP2 as candidate gene for a fatty acid composition QTL in porcine chromosome 8. Journal of Animal Breeding and Genetics 126, 52-8.
Fernandez A., Rodriganez J., Toro M.A., Rodriguez M.C. & Silio L. (2002) Inbreeding effects on the parameters of the growth function in three strains of Iberian pigs. Journal of Animal Science 80, 2267-75.
Gastaldi M., Diziere S., Defoort C., Portugal H., Lairon D., Darmon M. & Planells R. (2007) Sex-specific association of fatty acid binding protein 2 and microsomal triacylglycerol transfer protein variants with response to dietary lipid changes in the 3-mo Medi-RIVAGE primary intervention study. American Journal of Clinical Nutrition 86, 1633-41.
Green P., Falls K. & Crooks S. (1990) Documentation for crimap. Unpublished mimeo. Available at: http://linkage.rockefeller.edu/s...- ouverture dans une nouvelle fenêtre.
van Greevenbroek M.M., Robertus-Teunissen M.G., Erkelens D.W. & de Bruin T.W. (1998) Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in Caco-2 cells: interaction with saturated and unsaturated dietary fatty acids. Journal of Lipid Research 39, 173-85.
Hussain M.M., Shi J. & Dreizen P. (2003) Microsomal triglyceride transfer protein and its role in apoB-lipoprotein assembly. Journal of Lipid Research 44, 22-32.
Kahn R.A., Le Bouquin R., Pinot F., Benveniste I. & Durst F. (2001) A conservative amino acid substitution alters the regiospecificity of CYP94A2, a fatty acid hydroxylase from the plant Vicia sativa. Archives of biochemistry and biophysics 391, 180-7.
Ledmyr H., Karpe F., Lundahl B., McKinnon M., Skoglund-Andersson C. & Ehrenborg E. (2002) Variants of the microsomal triglyceride transfer protein gene are associated with plasma cholesterol levels and body mass index. Journal of Lipid Research 43, 51-8.
Ledmyr H., McMahon A.D., Ehrenborg E., Nielsen L.B., Neville M., Lithell H., MacFarlane P.W., Packard C.J. & Karpe F. (2004) The microsomal triglyceride transfer protein gene-493T variant lowers cholesterol but increases the risk of coronary heart disease. Circulation 109, 2279-84.
Letunic I., Copley R.R., Pils B., Pinkert S., Schultz J. & Bork P. (2006) SMART 5: domains in the context of genomes and networks. Nucleic Acids Research 34, D257-60.
Lichtenstein A.H. (2006) Thematic review series: patient-oriented research. Dietary fat, carbohydrate, and protein: effects on plasma lipoprotein patterns. Journal of Lipid Research 47, 1661-7.
Linding R., Jensen L.J., Diella F., Bork P., Gibson T.J. & Russell R.B. (2003) Protein disorder prediction: implications for structural proteomics. Structure 11, 1453-9.
Lu S., Huffman M., Yao Y., Mansbach C.M. II, Cheng X., Meng S. & Black D.D. (2002) Regulation of MTP expression in developing swine. Journal of Lipid Research 43, 1303-11.
Lundahl B., Skoglund-Andersson C., Caslake M., Bedford D., Stewart P., Hamsten A., Packard C.J. & Karpe F. (2006) Microsomal triglyceride transfer protein -493T variant reduces IDL plus LDL apoB production and the plasma concentration of large LDL particles. American Journal of Physiology. Endocrinology and Metabolism 290, E739-45.
Mourot J. & Hermier D. (2001) Lipids in monogastric animal meat. Reproduction, Nutrition, Development 41, 109-18.
Ovilo C., Fernandez A., Rodriguez M.C., Nieto M. & Silio L. (2006) Association of MC4R gene variants with growth, fatness, carcass composition and meat and fat quality traits in heavy pigs. Meat Science 73, 42-7.
Perez-Enciso M. & Misztal I. (2004) Qxpak: a versatile mixed model application for genetical genomics and QTL analyses. Bioinformatics 20, 2792-8.
Perez-Enciso M., Clop A., Noguera J.L. et al. (2000) A QTL on pig chromosome 4 affects fatty acid metabolism: evidence from an Iberian by Landrace intercross. Journal of Animal Science 78, 2525-31.
Ramensky V., Bork P. & Sunyaev S. (2002) Human non-synonymous SNPs: server and survey. Nucleic Acids Research 30, 3894-900.
Read J., Anderson T.A., Ritchie P.J., Vanloo B., Amey J., Levitt D., Rosseneu M., Scott J. & Shoulders C.C. (2000) A mechanism of membrane neutral lipid acquisition by the microsomal triglyceride transfer protein. Journal of Biological Chemistry 275, 30372-7.
Riquet J., Laval G., Iannuccelli N. et al. (2006) Identification of pig chromosomal regions under selection by a molecular whole genome scan analysis of the Taizumu SinoEuropean synthetic line. 8th WCGALP, Belo Horizonte (Brazil).
Rubin D., Helwig U., Pfeuffer M. et al. (2006) A common functional exon polymorphism in the microsomal triglyceride transfer protein gene is associated with type 2 diabetes, impaired glucose metabolism and insulin levels. Journal of Human Genetics 51, 567-74.
Serra X., Gil F., Perez-Enciso M., Oliver M.A., Vazquez J.M., Gispert M., Diaz I., Moreno F., Latorre R. & Noguera J.L. (1998) A comparison of carcass, meat quality and histochemical characteristics of iberian (guadyerbas line) and landrace pigs. Livestock Production Science 56, 215-23.
Swift L.L., Kakkad B., Boone C., Jovanovska A., Jerome W.G., Mohler P.J. & Ong D.E. (2005) Microsomal triglyceride transfer protein expression in adipocytes: a new component in fat metabolism. FEBS Letters 579, 3183-9.
Wood J.D., Enser M., Fisher A.V., Nute G.R., Sheard P.R., Richardson R.I., Hughes S.I. & Whittington F.M. (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343-58.
Zhao H., Rothschild M.F., Fernando R.L. & Dekkers J.C. (2003) Tests of candidate genes in breed cross populations for QTL mapping in livestock. Mammalian Genome 14, 472-82.
|
Language: | English.
|
Document Type: | Articles.
|
Journal Subset: | Life Sciences. Science.
|
ISSN: | 0268-9146
|
NLM Journal Code: | 4we, 8605704
|
DOI Number: | https://dx.doi.org/10.1111/j.136...- ouverture dans une nouvelle fenêtre
|
Annotation(s) | |
|
|