Information de reference pour ce titreAccession Number: | 00005797-200508030-00010.
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Author: | Hendriks, Edward F. 2,3; Matthews, Keith R. 1,3,*
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Institution: | (1)Institute of Immunology and Infection Research, School of Biological Sciences, Ashworth Laboratories, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK. (2)Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Flowers Building Room 3.21, South Kensington Campus, Imperial College London, London SW7 2AZ, UK. (3)School of Biological Sciences, Division of Biochemistry, 2.205 Stopford Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.
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Title: | |
Source: | Molecular Microbiology. 57(3):706-716, August 2005.
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Abstract: | Summary: The regulation of differentiation is particularly important in microbial eukaryotes that inhabit multiple environments. The parasite Trypanosoma brucei is an extreme example of this, requiring exquisite gene regulation during transmission from mammals to the tsetse fly vector. Unusually, trypanosomes rely almost exclusively on post-transcriptional mechanisms for regulated gene expression. Hence, RNA binding proteins are potentially of great significance in controlling stage-regulated processes. We have previously identified TbZFP1 as a trypanosome molecule transiently enriched during differentiation to tsetse midgut procyclic forms. This small protein (101 amino acids) contains the unusual CCCH zinc finger, an RNA binding motif. Here, we show that genetic ablation of TbZFP1 compromises repositioning of the mitochondrial genome, a specific event in the strictly regulated differentiation programme. Despite this, other events that occur both before and after this remain intact. Significantly, this phenotype correlates with the TbZFP1 expression profile during differentiation. This is the first genetic disruption of a developmental regulator in T. brucei. It demonstrates that programmed events in parasite development can be uncoupled at the molecular level. It also further supports the importance of CCCH proteins in key aspects of trypanosome cell function.
Copyright (C) 2005 Blackwell Publishing Ltd.
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Language: | English.
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Document Type: | Research Article.
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Journal Subset: | Life Sciences.
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ISSN: | 0950-382X
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NLM Journal Code: | mom, 8712028
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