Information de reference pour ce titreAccession Number: | 00008572-200205030-00006.
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Author: | Mellema, Stefan 1; Eichenberger, Waldemar 2; Rawyler, Andre 1; Suter, Marianne 1; Tadege, Million 1,+; Kuhlemeier, Cris 1,*
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Institution: | (1)Institute of Plant Sciences, Altenbergrain 21, CH-3013 Bern, Switzerland (2)Department of Chemistry and Biochemistry, CH-3012 Bern, Switzerland
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Title: | |
Source: | Plant Journal. 30(3):329-336, May 3, 2002.
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Abstract: | Summary: Rapid pollen tube growth requires a high rate of sugar metabolism to meet energetic and biosynthetic demands. Previous work on pollen sugar metabolism showed that tobacco pollen carry out efficient ethanolic fermentation concomitantly with a high rate of respiration ( Bucher et al., 1995). Here we show that the products of fermentation, acetaldehyde and ethanol, are further metabolised in a pathway that bypasses mitochondrial PDH. The enzymes involved in this pathway are pyruvate decarboxylase, aldehyde dehydrogenase and acetyl-CoA synthetase. Radiolabelling experiments show that during tobacco pollen tube growth label of 14C-ethanol is incorporated into CO2 as well as into lipids and other higher molecular weight compounds. A role for the glyoxylate cycle appears unlikely since activity of malate synthase, a key enzyme of the glyoxylate cycle, could not be detected.
(C) 2002 John Wiley & Sons, Ltd
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Author Keywords: | fermentation; ethanol; PDH-bypass; ADH; tobacco pollen; sugar metabolism.
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Language: | English.
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Document Type: | Original Articles.
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Journal Subset: | Science.
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ISSN: | 0960-7412
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NLM Journal Code: | bru, 9207397
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