Information de reference pour ce titreAccession Number: | 00001721-199901000-00004.
|
Author: | Harrison, P.; Robinson, M. S. C.; Mackie, I. J.; Joseph, J.; McDonald, S. J.; Liesner, R.; Savidge, G. F.; Pasi, J.; Machin, S. J.
|
Institution: | P. Harrison, M. S. C. Robinson, I. J. Mackie, J. Joseph, S. J. Machin and S. J. McDonald are from the Haemostasis Research Unit, Haematology Department, University College London; R. Liesner is with the Department of Haematology, Great Ormond Street Hospital for Sick Children, London; G. F. Savidge is with the Haemophilia Centre, St Thomas' Hospital, London; J. Pasi is with the Haemophilia Centre, Royal Free Hospital, London, UK.
|
Title: | |
Source: | Blood Coagulation & Fibrinolysis. 10(1):25-32, January 1999.
|
Abstract: | The PFA-100(R) device is a new instrument for the in-vitro testing of platelet function. Primary haemostasis is stimulated by recording the closure time taken for platelets to seal a 150 [mu]m aperture in the centre of a membrane coated with collagen and either epinephrine or ADP. Patients with type 3 von Willebrand's disease (n = 4) all had infinitely prolonged closure times (> 200 s) with both types of cartridge. A patient with afibrinogenemia exhibited only slightly prolonged closure times of 111 and 166 s for the ADP and epinephrine membranes, respectively. Patients with Glanzmann's thrombasthenia (n = 6) and Bernard Soulier syndrome (n = 2) had grossly prolonged closure times (> 200 s) with both types of cartridges. These results confirmed that the PFA-100(R) system was highly dependent on normal von Willebrand factor, glycoprotein lb and glycoprotein IIb/IIIa levels but not on plasma fibrinogen. Patients with storage pool disease (n = 6) and Hermansky Pudlak syndrome (n = 7) had prolonged closure times with the epinephrine cartridge. There was no evidence of enhanced platelet function in patients with antiphospholipid syndrome, in sickle-cell disease or thalassemia. However, ingestion of aspirin resulted in a near consistent and significant prolongation of the closure time for the epinephrine cartridge but not for the ADP cartridge in both normal subjects and patients. The test offers a reliable, reproducible, rapid and simple means of assessing high-shear platelet function in vitro.
(C) 1999 Lippincott Williams & Wilkins, Inc.
|
Author Keywords: | platelets; bleeding time; PFA-100(R); platelet disorders; von Willebrand's disease.
|
Language: | English.
|
Document Type: | Article: PDF Only.
|
Journal Subset: | Clinical Medicine.
|
ISSN: | 0957-5235
|
NLM Journal Code: | a5j, 9102551
|
Annotation(s) | |